PCR product table
Load a DNA sequence and primers, then click Perform PCR.
Drag expected fragments to the gel
The 50 bp ladder is shown in lane L. Drag each predicted PCR product into the matching sample lane. Smaller fragments migrate farther down the gel.
Fragment bank
Drag predicted amplicons into lanes 1–5.
L
Individual 1
Individual 2
Individual 3
Individual 4
Individual 5
50 bp ladder
Individual 1
Individual 2
Individual 3
Individual 4
Individual 5
How the simulator interprets PCR
The app searches both DNA strands. A valid product requires two primers facing each other on opposite strands. Amplicon length is calculated from the outer primer binding positions. This is a teaching simulator and does not evaluate melting temperature, primer dimers, mismatches, salt concentration, or polymerase-specific conditions.
